Stereological methods for biological morphometry software

Practical applications of morphometric analysis in toxicology. Although the cervical spinal cord csc of the horse has particular importance in diseases of cns, there is very little information about its segmental morphometry. A comparison of methods, ajp lung cellular and molecular physiology on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Characterization of these quantitative changes provides data that is useful not only for making a. Mar 15, 2010 besides biological variation, parameters tested included two variations of stereological methods unbiased counting frame versus 2ddisector, two sampling schemes few large versus frequent small sampling boxes, and workstations with varying degrees of sophistication. Software for morphometry images of biological drugs a powerful universal tool, which allows directly to control the process of inputting images, scanning products, as well as methods to automate routine image analysis of biological products and samples. Section 1 gives an overview of stereology, sections 2 point counting programs and 3 digitizing programs describe software currently being used for collecting raw data and estimating simple stereological parameters, and section 4 includes a discussion of several specialpurpose programs that offer access to some of the more advanced methods. Methods defined in this technique are essential for proper understanding of the structure of tissue, cells, cellular organelles, and other particles in the body of living organisms. The aim of this study was to compare the accuracy of digital image analysis tools and the.

Application of morphometric and stereological techniques on analysis and modelling of the avian lung, new insights into morphometry studies, pere m. This site is dedicated to helping researchers understand the principles of designbased stereology and its advantages over less sophisticated approaches in quantitative histology. In vivo stereological assessment of human cerebellar volume. This has caused the method of construction to be rethought as well as methods of restoration. In the present report, we apply this stereological method to evaluate the effects of age and gender on human cerebellar volume. Objective to assess the relationship between in vivo measurements of optic disc topography and histomorphometric measurements of optic nerve fiber number in glaucoma.

Stereological methods have been adapted to biological samples and are comprehensively described by weibel. The opportunities of confocal microscopy applied to morphometry of microscopical structures are presented and demonstrated on stereological methods based on evaluation of optical sections within a. This barcode number lets you verify that youre getting exactly the right version or edition of a book. Shtereom i is a simple windowsbased software for stereological estimation. The segmental morphometric properties of the horse cervical. This term is widely used in the stereological literature, which we have made an effort to respect in order to support a standardized terminology. All estimates were validated against exhaustive counts of the same nerve. Stereology is a powerful, efficient, and reliable set of methods for analyzing the morphology of the lung quantitatively ochs, 2006. The objective of the present study was to determine the morphometric features of the csc segments in the horse and possible relationships among the morphometric features. Stereological methods have evolved from being cumbersome and timeconsuming to being efficient and practical because of combined advances in stereological theory, sampling techniques, software, and imaging.

Changes induced by physical activity and weight loss in the. Sep 23, 2016 the large size of human tissues requires a practical stereological approach to perform a comprehensive analysis of the whole organ. Biological sciences chemical weapons analysis design and construction. Despite already available digital methods, the pathologists visual score is still widely considered as ground truth and used as a primary method in histomorphometric evaluations. Aug 27, 2009 by means of the cast software visiopharm, denmark, the stereological probes points, lines, and counting frames were superimposed onto the video images of the tissue sections viewed on the monitor.

Stereology is a completely different enterprise from computed tomography. In response to the intervention, there were substantial reductions in weight, bmi, and fat mass. The use of the letter q to refer to the intercepts of linear features with an area probe originates from the german term querschnitt originally used by weibel in his pioneering work involving the application of stereological methods to biological tissue weibel 1979. Feb 15, 2014 read alterations of mouse lung tissue dimensions during processing for morphometry. Then you can start reading kindle books on your smartphone, tablet, or computer no kindle device required. Enter your mobile number or email address below and well send you a link to download the free kindle app. Automated segmentation and morphometry of cell and tissue structures.

The large size of human tissues requires a practical stereological approach to perform a comprehensive analysis of the whole organ. A computed tomography algorithm effectively reconstructs. Some of the stereological systems now integrate spatial mapping with stereological analyses to provide more comprehensive methods to analyze brain tissue. Novel efficient methods for measuring mesophyll anatomical. Results for stereological parameters of the norway spruce needles and their mesophyll anatomical structure obtained using this studys novel stereological methods are shown in table 1. Stereological studies demonstrated that the sand was obtained from dunes facing the bays. Alterations of mouse lung tissue dimensions during.

Stereological analyses of the whole human pancreas. The statistical and mathematical principles used in stereology guarantee that no assumptionsor biasare introduced in the analysis. Use the link below to share a fulltext version of this article with your friends and colleagues. The incorporation of data archiving permits easier access to previous studies, as well as the sharing of stereological findings and their related maps throughout the scientific community. Calibration of the stereological estimation of the number of. Stereological procedures which allow the assessment of volume percentages, surfaces, lengths, numbers, and diameters are discussed in the light of dimension reduction. Whereas a polished rock surface is really a 2dobject, tissue sections will be 3d by their translucency. They are recommended reading prior to embarking upon a project requiring morphometric or stereologic analysis methods. Stereology some systems have been announced to do automatic stereology like c. Much of their practical application today is in the field of metallurgy and modifications of the techniques are used in ecology, geography, and earth sciences.

Actually, they were first devised as a method of analyzing the mineral composition of various rocks. The sections were evaluated using cavalieris method and volume fraction approach. Quantification of myocardial fibrosis by digital image. Software currently in active development the following software is in betatesting phase and as such it may still contain bugs relevant for proper program function. The application of stereological methods for estimating. Practical methods for biological morphometry vol 1 by weibel, ewald r. Mar 01, 2007 stereology has been earlier defined by wiebel 1970 to be. In despite of the argument about nomenclature morphometry and stereology could denominate the same method aherne and dunnill 1982 most authors consider morphometry a twodimensional.

One of the wellknown exceptions is imagej software 1, which is a public domain java image. Everyday low prices and free delivery on eligible orders. Tips for studies with quantitative morphology morphometry. Automated segmentation and morphometry of cell and tissue. Using these methods, differences can be demonstrated which are missed with the routine subjective methods. The comparison of selected mesophyll parameters was conducted to evaluate the effect of simulated acid rain on current norway spruce needles. Morphometrics from greek morphe, shape, form, and metria, measurement or morphometry refers to the quantitative analysis of form, a concept that encompasses size and shape. Final versions of each program as well as full support and windows compatibility will be available soon, along with the relevant publications. The segmented csc from five mature animals was used. Is length an appropriate estimator to characterize pulmonary. Most of stereological software is just a part of multifunctional software packages and, therefore, could incur very significant expenses that can interfere with the widespread use of computerized stereological tools. Stereology the definitive source for stereology information. Relationship of optic disc topography to optic nerve fiber. Morphometric applications in anatomic pathology, human.

We will consider how computers are being used to solve several problems of data management and to identify software packages that currently offer new and improved strategies for biological stereology. Morphometric analyses are commonly performed on organisms, and are useful in analyzing their fossil record, the impact of mutations on shape. When correctly implemented, the sensitivity and efficiency of stereological methods ensures accurate estimates with a fixed and predetermined precision. We have developed a method to quantitatively analyze the whole. Here, we present a stereological method, the optical fractionator, used to quantify the formation of new neurons, and their survival, in the rat hippocampus following electroconvulsive stimulation. The text by misell 1978 was written prior to the currently available software, but deals extensively with the principles and methods for image enhancement and analysis not confined to computers. Gene, cell and tissue basic application of stereology in. An official research policy statement of the american. The set of stereological morphometric grids macros for imagej. Cardiac fibrosis disrupts the normal myocardial structure and has a direct impact on heart function and survival. The optical fractionator technique to estimate cell numbers. There were significant improvements in aerobic fitness vo 2 max, insulin sensitivity, and fat oxidation after an overnight fast, as reflected in a decrease in fasting rq. Stereological tools in biomedical research 471 table i morphometry vs. Using these two stereological techniques, the mean glomerular volume and the total glomerular number per kidney were determined in j 6 kidneys of 8 rats.

Shtereom i simple windowsr based software for stereology. Methods both eyes of 10 monkeys macaca fascicularis with laserinduced glaucoma in the right eye were studied. Computer programs for biological stereology springerlink. Stereological techniques are not limited to the biological sciences.